Neutrophil Specific Antigen 1 (NB1)

NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia .Independently, Kissel et al. (2001) cloned and characterized NB1, which they also referred to as CD177 and HNA2A. They obtained the cDNA after purification and microsequence analysis of NB1 protein from normal resting granulocytes. Kissel et al. (2001) noted amino acid differences between the PRV1 (Temerinac et al., 2000) and NB1 sequences at positions 3, 119, 323, and 379 and suggested that 2 different, highly homologous genes may exist.By genomic sequence analysis and PCR, Kissel et al. (2002) determined that the NB1 gene contains 9 exons.