Poliovirus Receptor Related Protein 3 (PVRL3)

Reymond et al. (2000) cloned a full-length cDNA encoding PRR3. The deduced 550-amino acid PRR3 protein has a calculated molecular mass of 55.4 kD and contains an N-terminal hydrophobic leader sequence; an ectodomain containing a V-type Ig domain and 2 C-type Ig domains bracketed by 6 cysteine residues; a hydrophobic transmembrane region; and a long cytoplasmic sequence. PRR3 also has 6 potential N-glycosylation sites. Northern blot analysis revealed PRR3 expression predominantly in placenta and testis, with faint or no expression in other tissues. A major transcript of 2.8 kb and minor transcripts of 9.5, 6.0, and 4.7 kb were detected in placenta. PRR3 was also expressed in mammalian epithelial cell lines. Flow cytometry showed expression of PRR3 at the surface of transfected COS-1 cells.