Centromere Protein 32 (CENP32)

A second new kinetochore-associated protein, CENP-32/C9orf114, was sorted cluster diagram next to CLASP1 and CLASP2, two paralogues known to be involved in the regulation of microtubule dynamics. Like CLASPs, CENP-32/C9orf114 mapped to the outer kinetochore, and its depletion caused a significant accumulation of cells in later prometaphase with misaligned chromosomes. CENP-32 clusters with CLASP1 and CLASP2. A yeast homolog of CENP-32 interacts with CBF5, an enzyme involved in the posttranscriptional modification of rRNA, that was shown to bind to budding yeast centromeres and microtubules. CENP-32 is a memberof theSPOUTfamily of methyltransferases but is atypical in possessing a possible RNA-binding OB fold inserted into its catalytic domain.CENP-32 may function at kinetochores by interacting with an as-yet unknown RNA.