Eukaryotic Translation Initiation Factor 3M (EIF3M)

HFLB5 encodes a broadly expressed protein containing putative membrane fusion domains that acts as a receptor or coreceptor for entry of herpes simplex virus (HSV) The predicted 374-amino acid type II membrane protein contains 2 N-glycosylation sites and a C-terminal heptad repeat that forms a coiled-coil structure. HFLB5 lacks a signal peptide. Microscopic, immunoprecipitation, Western blot, and FACS analyses demonstrated that the HFLB5 C terminus is exposed extracellularly on the cell surface. Northern blot and RT-PCR analyses of human tissues and cell lines revealed broad expression of a 1.3-kb HFLB5 transcript. Entry of HSV into HFLB5-expressing porcine and human cells could be blocked by a 30-mer peptide identical to the HFLB5 C-terminal coiled-coil region.