G Protein Coupled Receptor 64 (GPR64)

GPR64 contains a signal peptide, followed by the variable region, a region with 11 conserved cysteines, a mucin-like serine/threonine/proline-rich sequence, and a C-terminal 7-transmembrane domain. A proteolysis site after amino acid 606 creates a hydrophilic N-terminal ectosubunit and a hydrophobic endosubunit.The cDNA encodes a 1,014-amino acid polypeptide. The predicted protein contains 7 transmembrane-spanning domains typical of G protein-coupled receptors and has an extended extracellular N terminus with similarity to highly glycosylated cell surface molecules such as mucin. HE6 transcript was abundant in the epididymis, but not found in any other tissue examined. In situ hybridization localized the transcript to the epithelial cells lining the epididymal duct. Southern blot analysis showed that HE6 is a single-copy gene.