Nuclear Receptor 2C2 Associated Protein (NR2C2AP)

Using TR4 in a yeast 2-hybrid screen of a testis cDNA library, followed by 5-prime RACE, Yang et al. (2003) cloned TRA16. The deduced 139-amino acid protein has a calculated molecular mass of 16 kD. Northern blot analysis detected a transcript of about 1.0 kb in all tissues examined, with highest expression in heart, skeletal muscle, and pancreas. Immunofluorescence microscopy detected endogenous TRA16 predominantly in nuclei of COS-1 cells and in several human cell lines.By coimmunoprecipitation of a transfected nonsmall cell lung carcinoma cell line, Yang et al. (2003) confirmed interaction between TRA16 and TR4. Electrophoretic mobility shift assay suggested that TRA16 may suppress binding between TR4 and the TR4 response element in target genes. Yang et al. (2003) also presented evidence that TRA16 blocks TR4 dimerization.