17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14)

17-beta-hydroxysteroid dehydrogenases, such as HSD17B14, are primarily involved in metabolism of steroids at the C17 position and also of other substrates, such as fatty acids, prostaglandins, and xenobiotics.The deduced 270-amino acid protein has a calculated molecular mass of 28.0 kD. It has all the characteristics of SDRs, including a cofactor-binding Rossman-fold domain and highly conserved motifs in the nucleotide-binding site and active site. Northern blot analysis detected HSD17B14 expression in liver, kidney, and retina.recombinant human DHRS10 converted NAD+ to NADH in the presence of estradiol, testosterone, or 5-androstene-3-beta,17-beta diol. Nonsaturable kinetics were found for testosterone, and no conversion was observed in the presence of NADPH.