2-Hydroxyacyl Coenzyme A Lyase 1 (HACL1)

The composite cDNA sequence of the human lyase contained an open reading frame encoding a predicted 578-amino acid polypeptide with a calculated molecular mass of 63,732 Da. Recombinant human protein expressed in mammalian cells exhibited lyase activity. 2-HPCL displays homology to a putative C. elegans protein that resembles bacterial oxalyl-CoA decarboxylases. Similar to the decarboxylases, a thiamine pyrophosphate-binding consensus domain is present in the C-terminal part of 2-HPCL. Although neither peroxisome targeting signal-1 nor -2 were apparent, transfection experiments with constructs encoding green fluorescent protein fused to the full-length lyase or its C-terminal pentapeptide indicated that the C terminus of 2-HPCL represents a peroxisome targeting signal-1 variant.