5"-Nucleotidase, Cytosolic IA (NT5C1A)

Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates.The predicted 368-amino acid protein has a calculated molecular mass of 47.7 kD.It is 83% identical to the pigeon protein, and both proteins contain a Walker B motif, 3 kinase-2 motifs, a kinase-3A motif, and 3 nucleoside monophosphate-binding motifs. Northern blot analysis detected a 10.0-kb transcript that was highly expressed in skeletal muscle, with lower expression in heart, brain, and pancreas, and much lower expression in kidney, testis, and uterus.ADP was required for full NT5C1A activity. Increased expression of active NT5C1A in cell lines led to resistance to several nucleoside analogs.