A Disintegrin And Metalloprotease 30 (ADAM30)

The ADAM30 EST encodes a polypeptide with sequence similarity to the cysteine-rich region of ADAM21. Cerretti et al. (1999) screened a human testis cDNA library with the ADAM30 EST and isolated cDNAs encoding 2 forms of ADAM30 that differ in the cytoplasmic domain. The first predicted ADAM30 protein has 790 amino acids and contains all of the domains characteristic of ADAMs. The metalloproteinase domain of ADAM30 has a consensus zinc-binding motif, suggesting that ADAM30 is proteolytically active. The second form of ADAM30, which the authors called ADAM30-beta, has a deletion of 9 amino acids in its cytoplasmic domain compared to the first form, resulting in a protein with 781 amino acids. Northern blot analysis of a variety of human tissues detected an approximately 3.0-kb ADAM30 transcript only in testis.