A Disintegrin And Metalloproteinase With Thrombospondin Like Protein 3 (ADAMTSL3)

Following removal of the signal peptide, the mature secreted protein has a calculated molecular mass of 186 kD. Punctin-2 has 16 putative N-glycosylation sites. Northern blot analysis detected a 7.5-kb mRNA that was expressed at high levels in heart, kidney, and liver, at moderate levels in skeletal muscle, small intestine, lung, and placenta, and at low levels in colon and spleen. No expression was detected in other tissues examined. Punctin-2 was expressed as a 7.5-kb transcript in mouse embryos in the second half of gestation. Glycosidase treatment confirmed that punctin-2 is a glycoprotein. Western blot analysis detected punctin-2 in the extracellular matrix, conditioned medium, and lysates of transfected COS-7 cells. Staining of the substratum following cell detachment confirmed extracellular matrix localization.