A Kinase Anchor Protein 8 Like Protein (AKAP8L)

AKAP8L shares 61% homology with AKAP95. Northern blot analysis showed ubiquitous and uniform expression of a 5.0-kb HA95 transcript. The N-terminal half of HA95 localized to the nucleus during interphase in a transfected human lymphoma cell line.The predicted protein has a calculated molecular mass of 72 kD, but SDS-PAGE showed an apparent molecular mass of about 100 kD. The N terminus of HAP95 contains both tandem and nontandem YG dipeptide repeats, followed by 3 FG repeats and a central nuclear localization signal. The C-terminal half contains 2 zinc finger motifs and 2 proline-rich SH3-binding domains. Northern blot analysis detected transcripts of 2.0 and 4.0 kb in all cell lines examined. Fluorescence-tagged HAP95 primarily localized to the nucleus of transfected HeLa cells, with exclusion from nucleoli.