ADP Dependent Glucokinase (ADPGK)

ADPGK catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions.Gel filtration analysis showed that the protein likely is monomeric. Database analysis showed expression in mammalian epithelial, endocrine, lymphatic, muscular, and organ tissues, consistent with a housekeeping function for Adpgk. Adpgk exhibited ADP-dependent glucokinase activity and had a bimodal optimal pH at 5.75 to 6.5 and 8.75 to 9.0. Activity was not inhibited by glucose-6-phosphate and other known modulators of glycolytic enzymes; however, high concentrations of glucose significantly inhibited enzyme activity.