ADP Ribosyltransferase 1 (ART1)

ADP-ribosylation is a reversible posttranslational modification used to regulate protein function. ADP-ribosyltransferases, such as ART1, transfer ADP-ribose from NAD+ to the target protein, and ADP-ribosylhydrolases reverse the reaction.Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. Okazaki et al. (1994) reported the nucleic acid and amino acid sequences of a human skeletal muscle ADP-ribosyltransferase. The human polypeptide shares 81.3% identity with rabbit skeletal muscle transferase. The 2 amino acid sequences predict predominantly hydrophobic N- and C-terminal regions, which are characteristic of glycosylphosphatidylinositol (GPI)-anchored proteins.