ATPase Family, AAA Domain Containing Protein 3A (ATAD3A)

ATAD3A contains a central transmembrane domain. ATAD3A was embedded in the inner membrane of HeLa cell mitochondria, with the C-terminal AAA domain directed toward the matrix and the N terminus exposed to the cytoplasm. its C-terminal half, the deduced 587-amino acid ATAD3A protein contains Walker A and B motifs, followed by ATP-binding sensor-1 and sensor-2 motifs. An alpha helix is located N-terminal to the Walker A motif, and an arginine finger separates the sensor motifs. An antibody that did not differentiate between ATAD3A and ATAD3B detected ATAD3 in a punctate pattern within mitochondria of human osteosarcoma cells. The pattern frequently coincided with mtDNA-containing nucleoids, but not all nucleoids contained ATAD3.