Acyl Coenzyme A Dehydrogenase Family, Member 8 (ACAD8)

ACAD8 cDNA containing a full-length coding sequence using RT-PCR on RNA from human adult brain and skin fibroblast. The predicted 415-amino acid ACAD8 protein contains many of the residues conserved in most other ACADs, including an active site glutamic acid residue and residues important for tetramer formation. Amino acid sequence identity between ACAD8 and other known ACADs range from 28% with GCDH to 38% with short-chain ACAD (ACADS). Northern blot analysis detected an approximately 2.1-kb ACAD8 transcript in all tissues examined, namely heart, lung, brain, skeletal muscle, pancreas, placenta, liver, and kidney, although it was weakly expressed in the last 2 tissues. RT-PCR showed ACAD8 expression in human cochlea and the myeloid cell line KG-1.