Adenylate Cyclase 8, Brain (ADCY8)

Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP.  Stimulatory or inhibitory receptors (Rs and Ri) interact with G proteins (Gs and Gi) that exhibit GTPase activity and they modulate the activity of the catalytic subunit of the adenylyl cyclase.Ca(2+) stimulation of ADCY8 occurs through loosely bound calmodulin. By mutation analysis of the N-terminal calmodulin-binding domain of mammalian Adcy8, Gu and Cooper (1999) determined that this domain makes a minor contribution to the Ca(2+) stimulation of Adcy8 activity, although it may be involved in calmodulin trapping. Mutagenesis and functional assays of the C-terminal calmodulin-binding site indicated that this site is largely responsible for the Ca(2+) stimulation of Adcy8.