Alcohol Dehydrogenase, Iron Containing 1 (ADHFE1)

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues.ADHFE1 encodes a deduced 467-amino acid protein with a predicted molecular mass of 50 kD. The protein shares 21 to 38% sequence identity with the type III ADHs of microbial organisms and 88%, 67%, and 53% identity with homologs in mouse, Drosophila, and C. elegans, respectively. Northern blot analysis detected a 2.4-kb transcript in liver only. MTC-panel screening PCR detected expression in adult liver, kidney, thymus, and spleen.Adhfe1 enzyme activity required both 4-hydroxybutyrate and alpha-ketoglutarate.