Ariadne Homolog 1 (ARIH1)

Sequence analysis predicted that the 557-amino acid protein, 72% identical to the Drosophila ariadne protein, contains a highly acidic domain and a poly(gly) tract in its N terminus, followed by 3 cys/his-rich motifs (2 RING fingers and an IBR, or """in between RING fingers""" domain) in its central region. Northern blot analysis detected ubiquitous expression of 2.2-, 2.7-, and 6.5-kb transcripts.The smallest transcript was weakly expressed except in testis, where it was predominant. Binding analysis showed that ARIH1, through a RING finger and its IBR motif, associates with at least the C-terminal 54 residues of UBCH7, as well as with UBCH8 (UBE2L6). ARIH1 did not associate with UBCH1 or UBCH5 (UBE2D1).