Carboxypeptidase A1, Pancreatic (CPA1)

Carboxypeptidase A1 is a monomeric pancreatic exopeptidase. It is involved in zymogen inhibition.The method allowed the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate the strategy, short diagnostic sequence tags (SSTs) were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1,000 tags revealed a gene expression pattern characteristic of pancreas. New pancreatic transcripts corresponding to novel tags were also identified. SAGE is based on 2 principles: first, that a short nucleotide sequence tag (9 to 10 bp) contained sufficient information to uniquely identify a transcript; and second, that concatenation of SSTs allows the efficient analysis of transcripts in a serial manner by the sequencing of multiple tags within a single clone.