Chymotrypsin Like Protein (CTRL)

One of these was located between the gene encoding the putative subunit of the proteasome complex (MECL1) and a protein serine kinase gene. The sequence of the protein was compared to protein sequences in the SWISSPROT database and identified as that of a chymotrypsin-like protease (CTRL). This gene has 7 exons, covers about 2 kb, and is transcribed in the same direction as the LCAT and the proteasome gene, from which it is immediately downstream.The open reading frame encodes a 264-amino acid proenzyme that contains a putative leader sequence of 18 amino acids. The sequence is 54% identical to that of human chymotrypsin B . Northern blot analysis showed that the gene is expressed specifically in the pancreas. The substrate specificity of the mature enzyme was distinct from that of chymotrypsin B.