Cytosolic Thiouridylase Subunit 1 (CTU1)

By sequencing proteins that interacted with URM1, followed by database analysis and PCR of a HeLa cell cDNA library, Schlieker et al. (2008) cloned ATPBD3. Endogenous ATPBD3 migrated at an apparent molecular mass of 38 kD by SDS-PAGE. Schlieker et al. (2008) showed that human ATPBD3 thiolated uracils on tRNAs with URM1 functioning as the sulfur carrier intermediate. Knockdown of either URM1 or ATPBD3 in HeLa cells reduced thiolation of uracil in the anticodon region of tRNA-lys(UUU) .Hartz (2009) mapped the ATPBD3 gene to chromosome 19q13.33 based on an alignment of the ATPBD3 sequence with the genomic sequence.