Deoxyribonuclease II Beta (DNASEIIb)

DLAD is a cation-independent acid endonuclease that shares homology with deoxyribonuclease II (DNASE2). Unlike DNASE2, however, DLAD shows a restricted tissue distribution.By searching an EST database for sequences similar to mouse Dlad, followed by 5-prime and 3-prime RACE of lung cDNA, Shiokawa and Tanuma (2001) cloned DLAD. The deduced protein contains 153 amino acids and includes a conserved active site histidine. Northern blot analysis of several tissues detected a 1.1-kb transcript only in lung. By genomic sequence analysis and PCR, Shiokawa and Tanuma (2001) identified a longer DLAD variant that encodes a deduced 361-amino acid protein with 5 putative N-glycosylation sites. The longer DLAD sequence shares 63.2% and 65.1% amino acid identity with mouse and rat Dlad, respectively.