Diacylglycerol Kinase Eta (DGKh)

DGKh encodes a member of the diacylglycerol kinase (DGK) enzyme family of proteins, specifically the type II DGK subfamily. Members of this family are involved in regulating the intracellular concentrations of diacylglycerol and phosphatidic acid. Two transcript variants encoding distinct isoforms have been identified for this gene.DGKH2 utilizes all 30 DGKH exons and encodes a deduced 1,220-amino acid protein with a calculated molecular mass of 135 kD. DGKH1, which lacks exon 29, encodes a deduced 1,164-amino acid protein has a calculated molecular mass of 128 kD. The 2 proteins differ at their C-terminal ends, and DGKH2 contains a SAM domain and a C-terminal PDZ-binding domain not found in DGKH1.Western blot analysis of transfected COS-7 cells detected DGKH1 and DGKH2 at apparent molecular masses of 128 and 135 kD, respectively