Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1)

ARGP1 was expressed in numerous human adult tissues and tissue culture cell lines, whereas expression of ARGP2 was more restricted. The ARGP1 cDNA encodes a protein of 488 amino acids with 9 predicted transmembrane domains, a potential N-linked glycosylation site, and a putative tyrosine phosphorylation motif. Comparison to ACAT1 revealed 22% amino acid identity over the entire molecule. Northern blot analysis of ARGP1 indicated ubiquitous expression with a great variability in level of expression. There was high expression in the adrenal cortex, adrenal medulla, testes, and small intestine, with moderate expression in thyroid, stomach, heart, skeletal muscle, and liver. A 2.0-kb transcript was invariable in all tissues examined, while a 2.4-kb transcript was observed in about half the tissues.