Enolase Superfamily, Member 1 (ENOSF1)

ENOSF1 was originally identified as a naturally occurring antisense transcript to the human thymidylate synthase gene. Other transcript variants (rTSbeta and rTSgamma) do not overlap the TS locus.The function of this gene appears to be primarily to regulate expression of the TS locus both via the antisense transcript as well as through the encoded proteins.All of TS exon 7 and part of intron 6 are overlapped by 3-prime rTS. Northern blot analysis of fractionated KB cells and other carcinoma cell lines revealed rTS transcripts of 1.8 and 6.3 kb, as well as several larger mRNA species. The presence and relative abundance of the larger transcripts varied between cell lines, and in vitro translation generated multiple translation products.