Exoribonuclease 3 (ERI3)

Using mouse prion protein (PRNP) as bait in a yeast 2-hybrid screen of a mouse brain cDNA library, Spielhaupter and Schatzl (2001) cloned Prnpip, which they called Pint1. The deduced protein contains 162 amino acids. Northern blot analysis detected a 3.8-kb Pint1 transcript in all mouse tissues examined. RNA dot blot analysis of mouse tissues revealed strong Pint1 expression in brain, heart, thyroid, and testis. Weak expression was detected in muscle cells, liver, pancreas, and kidney, and no expression was detected in mouse embryos or other tissues examined. By database analysis, Spielhaupter and Schatzl (2001) identified human PRNPIP.By immunoprecipitation of transfected baby hamster kidney cells, Spielhaupter and Schatzl (2001) identified mouse Pint1 in a complex with Prnp, synapsin Ib (SYN1), and the adaptor protein Grb2.