Glutaredoxin 3 (GLRX3)

It has an N-terminal thioredoxin (TXN) homology domain followed by tandem repeats of an 84-amino acid PICOT domain. Human PICOT shares 99% amino acid identity with the rat and mouse orthologs. The PICOT domain is highly conserved, and tandem PICOT domains are present in mouse and rat Picot. However, only a single PICOT domain is present in nematodes, yeast, bacteria, viruses, and plants. Northern blot analysis detected a 1.5-kb PICOT transcript in Jurkat cells. RT-PCR detected abundant PICOT expression in heart, spleen, and testis, with lower expression in other tissues. Western blot analysis detected PICOT at an apparent molecular mass of 38 kD. Fractionation of Jurkat cells revealed PICOT in the cytosolic compartment, with very low amounts in the membrane fraction.