JNK1/MAPK8 Associated Membrane Protein (JKAMP)

Jamp association with Jnk1 outcompetes Jnk1 association with Mkp5, resulting in increased and prolonged Jnk1 activity following stress. Elevated expression of Jamp following UV or tunicamycin treatment resulted in sustained Jnk1 activity and a higher level of Jnk1-dependent apoptosis, whereas inhibition of Jamp expression by siRNA reduced the degree and duration of Jnk1 activation and concomitantly the level of stress-induced apoptosis.Jamp contains 7 predicted transmembrane domains, a zinc finger domain, and an N-glycosylation site, which was confirmed experimentally. wide expression of Jamp in mouse tissues, including brain, spleen, thymus, liver, kidney, and testis. Use of a Flag epitope-tagged Jamp construct localized the protein primarily in the plasma membrane, but also in the endoplasmic reticulum and lysosomes.