Lysine Specific Demethylase 5B (KDM5B)

PLU1 encodes a deduced 1,544-amino acid protein containing 3 cysteine-rich zinc-binding PHD/LAP domains, a DNA-binding domain that is also found in the Drosophila dead ringer (dri) gene, 5 putative nuclear localization signals, and other conserved regions of unknown function. Northern blot analysis revealed expression of PLU1 transcripts at low levels in nonmalignant mammary cell lines and in colon cancer cell lines but at high levels in breast cancer cell lines. High expression in transformed breast cell lines decreased after treatment with 4D5. In situ hybridization analysis detected strong expression in the invasive but not in the benign components of primary breast carcinomas. Northern blot analysis detected high mRNA levels in normal testis and low levels in placenta, ovary, and tonsil.