Mannosyl Alpha-1,6-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT2)

MGAT2 is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. The predicted 447-amino acid GlcNAc-T II protein has the typical glycosyltransferase domains: a short (9-amino acid) N-terminal cytoplasmic domain, a 20-amino acid hydrophobic noncleavable signal-anchor domain, and a C-terminal catalytic domain. On Northern blots, GlcNAc-T II was transcribed as a major 3.0-kb mRNA, with minor bands at 2 and 4.5 kb, in all tissues tested.