Monooxygenase DBH Like Protein 1 (MOXD1)

The deduced 545-amino acid protein has several cysteine residues likely to be involved in disulfide bond formation. It also has a subset of cysteines within the catalytic core that are conserved with DBH and PAM, as well as other conserved residues that are likely to coordinate copper and contribute to enzymatic activity. The catalytic core regions of MOX and DBH share about 40% amino acid identity. Northern blot analysis detected a MOX transcript of about 3.3 kb in fibroblasts, and Northern blot analysis of several tissues showed highest expression in lung, kidney, and brain. RT-PCR analysis and RNA dot blot analysis confirmed highest expression in kidney and lung, and MOX was also expressed in testis, fetal kidney, uterus, and adrenal gland. Western blot analysis detected endogenous MOX at an apparent molecular mass of 62.6 kD.