N-Alpha-Acetyltransferase 15 (NaA15)

The deduced 866-amino acid protein has a calculated molecular mass of 101 kD. It contains 4 tetratricopeptide repeat domains in 2 tandems and a putative bipartite nuclear localization signal. NATH shares similarity with S. cerevisiae N-acetyltransferase-1 (NAT1) and mouse Narg1 and Tbdn1. Northern blot analysis detected a major transcript of 4.6 kb and a minor transcript of 5.8 kb in a papillary carcinoma cell line and in an anaplastic thyroid carcinoma cell line. Multitissue mRNA dot blot analysis showed low expression in most adult tissues and specific brain regions examined. Highest expression was detected in testis and a Burkitt lymphoma cell line. Expression of NATH in COS-7 cells resulted in cytoplasmic staining.The deduced proteins contained 865 and 815 amino acids. The shorter protein has an N-terminal truncation.