NADH Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 2 (NDUFaF2)

B17.2L shares 20% and 17% amino acid identity with yeast B17.2L and human B17.2, respectively. Western blot analysis detected B17.2L expression in human fibroblasts, heart, skeletal muscle, and liver. Immunoprecipitation of B17.2L from normal human heart mitochondria coimmunoprecipitated other complex I subunits.The deduced 169-amino acid protein has a calculated molecular mass of 19.9 kD. Mimitin contains a central domain found in the complex I B17.2 protein (NDUFA12) and a C-terminal ATP/GTP-binding site motif A. Human and mouse mimitin share 80% amino acid identity. Western blot analysis detected endogenous mimitin at an apparent molecular mass of 20 kD. Immunolocalization and Western blot analysis of fractionated cells confirmed that mimitin is a mitochondrial protein.