Phosphodiesterase 3A, cGMP Inhibited (PDE3A)

Cyclic nucleotide phosphodiesterases (PDEs) comprise a complex group of enzymes, and at least 5 major PDE families or classes with distinctive properties have been identified. Members of the cGMP-inhibited cAMP PDE (cGI-PDE) family, such as PDE3A, are characterized by high affinity for cAMP and cGMP and competitive inhibition of their cAMP hydrolytic activity by cGMP and certain positive inotropic agents .ORF encodes a predicted protein of 125 kD. A single mRNA species hybridized with a 4-kb EcoRI restriction fragment of the cDNA containing the entire ORF. The C-terminal region of the deduced amino acid sequence contains a putative catalytic domain conserved among mammalian PDE families.