Polymerase DNA Directed Epsilon 4 (POLe4)

POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner.These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging. The deduced protein contains 117 amino acids and has a calculated molecular mass of about 12 kD. POLE4 contains a histone-fold motif that shares 34% identity with the histone-fold motif of the DPB3 subunit of S. cerevisiae polymerase epsilon. In vitro translated POLE4 showed an apparent molecular mass of 19 kD by SDS-PAGE.The POLE3-POLE4 complex, but not POLE3 or POLE4 alone, could immunoprecipitate the in vitro translated 261-kD catalytic subunit (POLE1) and the 59-kD accessory subunit (POLE2) of the DNA polymerase epsilon complex.