Procollagen C Proteinase Enhancer 2 (PCPE2)

PCOLCE2 lies 5.2 kb and 102.5 kb centromeric to the transient receptor potential channel-1 (TRPC1) and plastin-1 (PLS1), respectively. The deduced 415-amino acid protein contains an N-terminal signal sequence, 2 CUB domains, and an NTR domain. It also contains a putative myristoylation site, several potential phosphorylation sites, a putative glycosylation site, and an RGD site. PCOLCE2 shares about 43% sequence identity with the PCOLCE protein .The secreted PCOLCE2 protein is calculated to have a molecular mass of about 47 kD. Northern blot analysis detected ubiquitous expression of a 2-kb transcript, with highest expression in heart, placenta, and trabecular meshwork, and very low expression in brain. Western blot analysis revealed a 52-kD form of PCOLCE2 in human fibroblast cells.