Protein Kinase C And Casein Kinase Substrate In Neurons 3 (PACSIN3)

Sequence analysis predicted that the 424-amino acid human PACSIN3 protein, which is 94% identical to the mouse protein, has an N-terminal CDC15 domain and a conserved C-terminal SH3 domain preceded by a proline-rich region. RNA dot blot analysis detected PACSIN3 expression mainly in skeletal muscle, heart, lung, kidney, adrenal gland, and placenta. Immunoblot analysis showed predominant expression of a 48-kD protein in mouse lung, skeletal muscle, and heart; expression was also detected in brain, kidney, and uterus. Immunofluorescence microscopy demonstrated colocalization of PACSIN3 with dynamin (DNM1) on the cytoplasmic side of the plasma membrane. GST pull-down analysis indicated binding to dynamin, synaptojanin (SYNJ1), and N-WASP (WASL) through an intact SH3 domain.