Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Inhibitor, Repressor Of (PRKRIR)

PRKRIR contains a region (amino acids 86 to 200) of limited homology (24% identity) to the charged domain of HSP90.The THAP domain of the deduced 761-amino acid THAP0 protein includes a C2CH signature, an AVPTIF box, and several other conserved amino acids.When coexpressed with PKR in yeast, P58(IPK) repressed PKR-mediated EIF2-alpha phosphorylation, inhibiting the normally toxic and growth-suppressive effects associated with PKR function. Conversely, introduction of P52(rIPK) into these strains resulted in restoration of both PKR activity and EIF2-alpha phosphorylation, concomitant with growth suppression due to inhibition of P58(IPK) function.