Protein Phosphatase 1, Regulatory Subunit 14B (PPP1R14B)

PNG had no striking similarity to other known genes at the DNA level, however, analysis of hybridization to a panel of somatic cell hybrids indicated the existence of related sequences on chromosomes 2, 4, 7, and 22. PNG showed expression of a 1-kb message in multiple tissues. The predicted protein is 119 amino acids long. The gene spans approximately 2.5 kb and is divided into 4 exons and 3 introns. It is located 4.4 kb upstream of PLCB3, with the 5-prime ends of each gene facing each other. The intragenic region showed separate CpG islands at each end separated by a stretch of 2 kb, characterized by periodic alteration of the GC content. A 5-prime flanking region of PNG did not contain TATA or CCAAT, suggesting to the authors a housekeeping promoter structure.