Protein Phosphatase, Mg2+/Mn2+ Dependent 1M (PPM1M)

Komaki et al. (2003) cloned mouse Pp2c-eta from a 13.5-day embryo cDNA library. The deduced 406-amino acid protein contains 6 motifs conserved in all PP2C family members, and it has a unique nuclear localization signal between motifs III and IV. By database analysis, Komaki et al. (2003) identified a cDNA encoding human PP2C-eta Northern blot analysis detected expression of Pp2c-eta in all mouse tissues examined. Strongest expression was in testis, followed by lung, kidney, brain, heart, and liver. Pp2c-eta localized mainly to the nucleus of transfected cells.Komaki et al. (2003) found that recombinant mouse Pp2c-eta expressed in E. coli exhibited Mn(2+)-dependent protein phosphatase activity against a test substrate.The International Radiation Hybrid Mapping Consortium mapped the PP2C-eta gene to chromosome 3