Pseudouridylate Synthase Like Protein 1 (PUSL1)

PUSL1, Belongs to the tRNA pseudouridine synthase truA family. This enzyme belongs to the family of isomerases, specifically those intramolecular transferases transferring other groups. The systematic name of this enzyme class is tRNA-uridine uracilmutase. TruA is a pseudouridine synthase that specializes in three positions in the tRNA, positions 38, 39, and 40. These positions are in the critical base-paired region adjacent to the anticodon. The enzyme is a dimer of two identical subunits, which embraces both the 38-40 region of the tRNA and regions farther up the molecule. The enzyme acts on 17 different tRNA molecules in the bacteria cell. It appears to prefer tRNA molecules that are flexible, passing up ones that already have rigid anticodon stem loops. In this way, the enzyme helps the cell fine tune its tRNA, making them rigid but not too rigid for optimal function.