RNA Methyltransferase Like Protein 1 (RNMTL1)

RNMTL1 shares 32 to 42% amino acid identity with S. cerevisiae Pet56 and E. coli RNA methyltransferases, especially over the region implicated in transfer of the methyl group to rRNA. RT-PCR analysis showed no difference in expression between human hepatocellular carcinoma and normal liver cell lines or tissues.Transfection of deletion mutants, followed by luciferase reporter assay, identified a minimal promoter element at -71 to +1 of the RNMTL1 sequence. Database analysis, followed by mobility shift assay, determined that the RNMTL1 promoter is bound by ATF/CREB, E2F-DP/SP1, and other transcription factors. YY1 acts as a negative regulator of RNMTL1 transcription via a proposed interaction with ATF/CREB.