Rho GTPase Activating Protein 25 (ARHGAP25)

ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases, which are implicated in actin remodeling, cell polarity, and cell migration. The deduced 638-amino acid protein shares similarity with CDC42GAP (ARHGAP1). Northern blot analysis detected highest expression in lung, spleen, and peripheral blood leukocytes. Expression was moderate in ovary and small intestine, weak in placenta, liver, skeletal muscle, kidney, thymus, and prostate, and not detected in heart, brain, pancreas, testis, and colon.The other variant contains an alternate promoter and encodes a 639-amino acid protein that differs from the KIAA0053 protein at the N terminus. Both isoforms have an N-terminal pleckstrin homology domain, followed by a RhoGAP domain and a C-terminal coiled-coil domain.