Rhophilin 1 (RHPN1)

The rho GTPase is involved in a signaling pathway that regulates cell adhesion and cytokinesis, among other activities. Watanabe et al. (1996) used the yeast 2-hybrid system to identify cDNAs that interact with rho. One clone was isolated from a mouse embryo cDNA library. A full-length sequence derived from several clones encodes a predicted 643-amino acid protein that they named rhophilin. Northern blots showed a 3.2-kb RNA was expressed at highest amounts in the testis.Watanabe et al. (1996) found that a region at the amino terminus of rhophilin shares sequence similarity with PKN and is the site for rho binding. PKN signaling stimulated AR activity in the presence of adrenal androgens and in the presence of an AR antagonist.