Serine/Threonine Kinase 36 (STK36)

STK36 coimmunoprecipitated with SUFU, GLI1, GLI2, and GLI3 in cotransfected 293 cells. In the presence of SUFU, about 3% of cells exhibited nuclear staining of GLI1 or GLI2. When STK36 was introduced, however, about 20% of cells possessed nuclear GLI1 or GLI2 staining.The deduced 1,311-amino acid protein shares about 55% identity over 795 amino acids with the Drosophila """fused""" (Fu) protein. RT-PCR ELISA detected expression of STK36 at low to moderate levels in most tissues tested, but not in adult skeletal muscle and fetal liver.The deduced 1,315-amino acid protein shares 55% homology with Drosophila Fu in the N-terminal kinase domain, but only limited homology over the remaining 1,052 amino acids. Highest expression was in testis.