T-Cell Activation Protein Phosphatase 2C (TAPP2C)

By microarray analysis to identify genes coregulated with interleukin-2 (IL2) in a combination of experiments involving T-cell activation and other conditions, followed by RT-PCR, Mao et al. (2004) cloned TAPP2C. The 304-amino acid protein contains a protein phosphatase-2C domain .The International Radiation Hybrid Mapping Consortium mapped the TAPP2C gene to chromosome 12.The structure revealed a novel protein fold with a catalytic domain composed of a central beta sandwich that binds 2 manganese ions, which is surrounded by alpha helices. The authors stated that the protein architecture and deduced catalytic mechanism are similar to the PP1, PP2A, and PP2B family of protein ser/thr phosphatases.