Ubiquitin Conjugating Enzyme E2T (UBE2T)

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3). This monoubiquitination was stimulated by the presence of FANCL and inactivated UBE2T. UBE2T activity was dependent on the conserved cys86, where a ubiquitin molecule is transferred from E1, and UBE2T underwent automonoubiquitination at lys91 in vivo. The monoubiquitination of UBE2T occurred even in the absence of an E3 in vitro.The deduced 197-amino acid protein contains a ubiquitin-conjugating enzyme active site. EST database analysis indicated widespread UBE2T expression.