Ubiquitin Specific Peptidase 6 (USP6)

TRE2, the complete cDNA spans 8,201 bp and possesses an unusually long noncoding region and a translatable region with 2 open reading frames (ORF). In 1 cDNA clone, the presence of 2 insertion sequences suggested the possibility of alternative splicing. Transfection-tumorigenicity assays with the ORFs subcloned into expression vectors were positive for the ORF adjacent to the 5-prime noncoding region and negative for the second, downstream ORF. Analysis of the 786-amino acid sequence deduced from the 5-prime ORF predicted a highly hydrophilic protein with 2 charge clusters suggesting nucleic acid-binding properties. When used as a probe, the cloned sequence detected RNA transcripts in a wide variety of human cancer cells regardless of their lineage of origin from different tissues, but not in human cells from normal tissue.