Zinc Phosphodiesterase ELAC Protein 1 (ELAC1)

By sequencing a region of chromosome 18 that is deleted in the Ma29 lung cancer cell line, followed by screening a HeLa cell cDNA library, Yanaihara et al. (2001) cloned ELAC1, which they called D29. The deduced protein contains 363 amino acids. The D29 transcript contains 2 polyadenylation sequences. Yanaihara et al. (2001) also identified an 88-amino acid variant of D29 that is translated from a different reading frame. Northern blot analysis detected a major transcript of 1.6 kb and minor transcripts of 1.2, 2.0, and 2.5 kb in most tissues examined. Transcripts of 4.0, 5.5, and 7.0 kb were also detected in pancreas. RT-PCR detected expression in all tissues examined.Yanaihara et al. (2001) determined that the D29 gene contains 4 exons.